Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ECM1 antibody at 4.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 47, 20, 64 kDa Observed band size: 47 kDa
IHC image of CSB-PA007383LA01HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Supplier Page from CUSABIO Technology LLC for ECM1 Antibody